The USCPA is concerned for the welfare of the mother of the puppies dumped close to Ballyconnell.
On Monday, three Labrador-cross pups were found on the Gortaree Road in Fermanagh, between Derrylin and the border.
One of the pups has since died, but the other two are said to be recovering well.
An appeal is being made for information as to how they ended up in a ditch.
Development Manager with USPCA, Colleen Tinnelly has been sharing the details and making an appeal with Sheila Naughton :